20 � Hydroxy Ecdysone - Exporter, Manufacturer, Distributor, Supplier, Trading Company, Thane, India

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Product Specification

20 Hydroxy Ecdysone
Odorless, off white-white amorphous powder
Solubility: alcohol, methanol, water If crystallized from water, mp 150-151°, plates from EtOAC: MeOH, mp 241-242° [α]n + 61.8°
UV (EtOH) 242 nm, IR (KBr) 3400(OH), 1653 cm^-1(enone)

Crustacean and insect molting hormone (i.e. 20-hydroxyecdysone) was isolated from seawater crayfish Jasus lalandei (JL-1), silk worm pupae Bombay mori, pupae of oak silk moth Antheraca pernyi, tobacco horn-worm Manduca Sexta in early sixties or seventy. [1]. Animal sources (especially Arthropods) are not the ultimate resources of ecdystroids. Now near about 300 different molecules related to ecdysone from thousands of different plant species has been systematically investigated. They are present in significant amounts in 5-6% of plant species investigated so far, their concentration ranging from 50 ng/g to as much as 30 mg/g dry mass (i.e. 3%) [2]
Plants usually contain one or a few major ecdysteroids together with a wide array of closely related molecules present in lower amounts and these molecules are throught to protect plants against non-adapted phytophagous insects.

Why the term Ecdysteroids has been coined?
(Greek, ecdysis =molt), ecdysteroids include insect molting hormones, which regulate insect development. Insect, crustaceans and other arthropods have tough cuticles or exo-skeletons which serve to support and contain their internal organs and muscles. As these cuticles are incapable and replaced by new ones. This process of molting or ecdysis allows the animal to grow and acquire adult characteristics. [1]

Biological-Medicine effects
Ecdysteroids increase protein synthesis in mammals without thymolytic, androgenic or antigonadothropic side effect .The scientific literature contains substantial evidence to support their effect on lipid metabolism. Ecdysteroids have been shown to decrease cholesterol levels, stimulate the immune system; potential the effect of insulin and normalized hyperglycemia, of key importance is the fact that ecdysteroids make the individual adaptogenic [3].

20-hydroxyecdysone- the principle ecdysteroids in invertebrate and plants.
In invertebrates and in plants, 20-hydroxyecdysone is the most frequently found and widely distributed ecdysteroids. Due to the accumulation of data from studies on it and its many practical applications, 20 hydroxyecdysone has gained remarkable significance among the ecdysteroids. Plants continue to be an optimal source for 20-hydroxyecdysone, That is, they supply the majority of raw materials required to conduct research on insect physiology, pre-clinical Pharmacognosy and receptor binding [4].

Analysis
Purity profile
The homogeneity of 20-hydroxyecdysone was controlled by various analytical procedures that included TLC and HPLC. Triple detections are employed to locate the dark spot (UV light at 254nm, 366nm and by derivatization). HPLC is a sophisticated and preferred method to control for 20 hydroxyecdysone purity [5,6]
Quantitative determination of 20 hydroxyecdysone by HPLC
Column : reverse phase C18e, 5µ
Mobile phase : Acetonitrile: Water (23:77) with 0.1% TFA
Wavelength : ?max 254

Our product specification
We are offering 50% 20- hydroxy ecdystrone enriched extract. Chromatogram of this is enclosed here.

References:

D.H.S.Horn (1971), The Ecdysones in Naturally Occurring Insecticides., M Jacobson and D.G.Gosby (ed). Marcel Dekker, N.Y., 333-459
Dinan. L et al. (2001). Chromatographic procedure for the isolation of plant steroids. J Chromatogr.A 935,105-123
Maria Bathori (1998). Purification and charecterization of plant ecdystroids of silene species. Trendsa in analytical chemistry. 17(6), 372-383
Maria Bathori (2000), the analysis and crystallographic charecterization of 20 hydroxyecdysone, Current Medicinal Chemistry, 7, 1305-1312
I.D.Wilson (1985), Thin-layer chromatography of ecdysteroids, J Chromatogr, 318-377
H.Kalasz et.al (2000), Two -dimensional thin layer chromatography of plant ecdystroids, Chromatographia, 52 (11/12), 815-817

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